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Evaluation Of The Detoxification Potential Of Lactic Acid Bacteria From Curd And Whey Against Ochratoxin A In Broiler

by Afshan shabbir | Ms Huma Mujahid | Dr. Asif Nadeem | Dr. Muhammad Tayyab.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1657,T] (1).

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Mutation Analysis Of Alpha-Synuclein Gene In Patients With Parkinson Disease

by Iffat Aleem (2009-VA-566) | Dr. Asif Nadeem | Prof. Dr. Tahir Yaqub | Ms. Huma Mujahid.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Parkinson disease is a complex, heterogeneous and chronic neurodegenerative disorder with a cumulative prevalence of greater than one per thousand, caused by neuronal loss, mainly affecting dopaminergic neurons of the substantia nigra. Parkinson disease is an idiopathic disorder of the extra pyramidal system characterized by tremors. Genetic factors contribute to its complex pathogenesis. A functional repeat polymorphism in the α-synuclein (SNCA) gene promoter conveys susceptibility for Parkinson disease. The α-synuclein (SNCA) has been implicated in rare autosomal dominant forms of Parkinson disease. The mutations in α-synuclein were associated with severe disease progression and a typical physical signs, indicative of neuro degeneration extending beyond the substantia nigra. Mutation in α-synuclein gene may have association with dopaminergic neuronal loss in Parkinson disease. Blood samples were collected from Parkinson disease patients. DNA was extracted by organic method. Primers were designed using Primer3 software. Amplification of gene was done by Polymerase Chain Reaction. PCR products were sequenced bi-directionally on ABI 3130XL Genetic analyzer. Sequence alignment was performed for polymorphism identification. The analysis of identified polymorphism has been done by CHROMAS software. Sequences were aligned by BLAST tool of NCBI. The results of analysis showed that no mutation found in exonic region of α-synuclein (SNCA) gene in Pakistani individuals selected for this study. Any change in exonic region of α-synuclein (SNCA) gene is a rare cause of sporadic and familial Parkinson disease in different populations. Availability: Items available for loan: UVAS Library [Call number: 2325-T] (1).

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Polymorphism Study Of Calcium-Sensing Receptor Gene (Casr)In Calcium Nephrolithiasis Affected Families

by Hafza Ammara (2013-VA-865) | Dr. Muhammad YasirZahoor | Dr. Asif Nadeem | Ms. Huma Mujahid.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Nephrolithiasis is a multi-factorial kidney stone disease resulting from the combined influence of epidemiological, biochemical and genetic risk factors. Calcium-sensing receptorprotien is plasma membrane G protein-coupled receptors that regulate secretion of parathyroid hormoneand calcium re-absorption by kidney tubular cells. This protienis able to sense small changes in circulating calcium concentration and, once activated, it inhibits parathyroid hormone secretion and renal tubule calcium re-absorption. The CaSR gene protein islocated on chromosome 3q13 is one of the candidate gene explaining individual predispositions to calcium nephrolithiasis. CaSR gene is a predecessor for nephrolithiasis due to its role in calcium re-absorption. CaSRgene has seven exons and several mutations have been reported globally related to calcium nephrolithiasis. Twenty families affected with calcium nephrolithiasis having at least two affected individuals have been enrolled for this study. Ten families have already been analyzed for exon 3 & 4 in the laboratory. DNA has been extracted through inorganic extraction method from the blood of newly enrolled families. Primers have been designed for exon 5, 6 and 7 through Primer3 software. These exons have been sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer/ABI) and have been read in an automated sequencer, ABI Prism model 3730 (Perkin Elmer). We also screend the coding exon of CLDN14 genewhich is a membrane protein that regulates paracellular passage of ions and small solutesat epithelial tight junction.The overexpression of claudin-14 in the thick ascending limb of loop of henleof the kidney generates a renal phenotype characteristic with hypomagnesemiaand hypercalciuria that leads to the development of calcium nephrolithiasis. All of the sequences have been evaluated by using Clustal-W programs, Chromas and Bioedit software for mutational analysis.Sequence analysis of CaSR gene revealed one novel splice mutationC>G at position 63722 at exon 5 in one affected family.This variation is found in the intronic region of the gene.We found one missense mutation Q536R at exon six in three different affected families. And one synonymous single nucleotide polymorphism(SNP) C>G found at exon 7at rs2036400 in six different affected families.These SNPs showsa significant association of CaSRgene with nephrolithiasis. It will help to determine the risk factor and role of CaSR gene in inheritance of calcium nephrolithiasis. And it will also be used for genetic screening and prenatal diagnosis. Availability: Items available for loan: UVAS Library [Call number: 2426-T] (1).

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